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    Boster Bio ek0411 mouse tnfα elisa kit boster biological technology
    Ek0411 Mouse Tnfα Elisa Kit Boster Biological Technology, supplied by Boster Bio, used in various techniques. Bioz Stars score: 96/100, based on 930 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/elisa+kit+boster+biological+technology/Mouse+IL-6%2FInterleukin-6+ELISA+Kit+PicoKine/pm41175872-242-148-153
    Average 96 stars, based on 930 article reviews
    ek0411 mouse tnfα elisa kit boster biological technology - by Bioz Stars, 2026-09
    96/100 stars

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    Bicinchoninic Acid Protein Assay:

    Article Title: Itaconate transporter SLC13A3 confers immunotherapy resistance via alkylation-mediated stabilization of PD-L1.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Fetal bovine serum Biological Industries Cat# 04-001-1ACS NAA Med Chem Express Cat# HY-118628 4-Octyl itaconate Med Chem Express Cat# HY-112675 Opti-MEM Gibco Cat# 31985-062 TRIzol Thermo Fisher Scientific Cat# 15596018 Cycloheximide (CHX) Aladdin Cat#C112766; CAS No. 66-81-9 Critical commercial assays BCA Protein Assay Kit Thermo Fisher Scientific Cat# 23225 PrimeScript RT Master Mix kit Takara Cat# RR036A Mouse IL-1b ELISA kit BOSTER Biological Technology Cat# EK0392 Mouse TNF-a ELISA kit BOSTER Biological Technology Cat# EK0527 Deposited data Raw RNAseq data This paper GSE280490 Data used to generate figures This paper Data S1 Raw data of WB This paper, Mendeley Data https://doi.org/10.17632/k6395ns5pk.1 Experimental models: Cell lines Human: HEK293T ATCC Cat# CRL-11268; RRID: CVCL_1926 Human: THP-1 ATCC Cat# TIB-202; RRID: CVCL_0006 Human: 786-O ATCC Cat# CRL-1932; RRID: CVCL_1051 Human: SW839 ATCC Cat#HTB-49;RRID: CVCL_3604 Human: A549 ATCC Cat#CCL-185;RRID: CVCL_0023 Mouse: Lewis ATCC Cat# CRL-1642; RRID: CVCL_4358 Mouse: Renca ATCC Cat# CRL-2947; RRID: CVCL_2174 Renca Slc13a3 KO cells This paper N/A Experimental models: Organisms/strains Mouse: C57BL/6J Xi’an Jiaotong University N/A Mouse: Slc13a3 KO C57BL/6J Cyagen Biosciences Inc N/A Mouse: Balb/c Xi’an Jiaotong University N/A Oligonucleotides Primers for qPCR analysis, see Table S1 This paper N/A Recombinant DNA pCMV-PD-L1-HA Addgene Cat# 121492 pCMV-8xHis-Ub Addgene Cat# 107392 pCMV-PD-L1 C272A-HA This paper N/A pMD2.G Addgene Cat# 12259 psPAX2 Addgene Cat# 12260 pCMV-PD-L1 C-terminal-GST This paper N/A pCMV-PD-L1 C-terminal C272A-GST This paper N/A Software and algorithms GraphPad Prism 8.0.2 Graphpad N/A FlowJo X software V10.0.07 FlowJo N/A ImageJ National Institutes of Health N/A Cell Metabolism 37, 1–13.e1–e5, February 4, 2025 e2 mice were housed and fed in a pathogen-free environment at the Laboratory Animal Center of Xi’an Jiaotong University. .. REAGENT or RESOURCE SOURCE IDENTIFIER Fetal bovine serum Biological Industries Cat# 04-001-1ACS NAA Med Chem Express Cat# HY-118628 4-Octyl itaconate Med Chem Express Cat# HY-112675 Opti-MEM Gibco Cat# 31985-062 TRIzol Thermo Fisher Scientific Cat# 15596018 Cycloheximide (CHX) Aladdin Cat#C112766; CAS No. 66-81-9 Critical commercial assays BCA Protein Assay Kit Thermo Fisher Scientific Cat# 23225 PrimeScript RT Master Mix kit Takara Cat# RR036A Mouse IL-1b ELISA kit BOSTER Biological Technology Cat# EK0392 Mouse TNF-a ELISA kit BOSTER Biological Technology Cat# EK0527 Deposited data Raw RNAseq data This paper GSE280490 Data used to generate figures This paper Data S1 Raw data of WB This paper, Mendeley Data https://doi.org/10.17632/k6395ns5pk.1 Experimental models: Cell lines Human: HEK293T ATCC Cat# CRL-11268; RRID: CVCL_1926 Human: THP-1 ATCC Cat# TIB-202; RRID: CVCL_0006 Human: 786-O ATCC Cat# CRL-1932; RRID: CVCL_1051 Human: SW839 ATCC Cat#HTB-49;RRID: CVCL_3604 Human: A549 ATCC Cat#CCL-185;RRID: CVCL_0023 Mouse: Lewis ATCC Cat# CRL-1642; RRID: CVCL_4358 Mouse: Renca ATCC Cat# CRL-2947; RRID: CVCL_2174 Renca Slc13a3 KO cells This paper N/A Experimental models: Organisms/strains Mouse: C57BL/6J Xi’an Jiaotong University N/A Mouse: Slc13a3 KO C57BL/6J Cyagen Biosciences Inc N/A Mouse: Balb/c Xi’an Jiaotong University N/A Oligonucleotides Primers for qPCR analysis, see Table S1 This paper N/A Recombinant DNA pCMV-PD-L1-HA Addgene Cat# 121492 pCMV-8xHis-Ub Addgene Cat# 107392 pCMV-PD-L1 C272A-HA This paper N/A pMD2.G Addgene Cat# 12259 psPAX2 Addgene Cat# 12260 pCMV-PD-L1 C-terminal-GST This paper N/A pCMV-PD-L1 C-terminal C272A-GST This paper N/A Software and algorithms GraphPad Prism 8.0.2 Graphpad N/A FlowJo X software V10.0.07 FlowJo N/A ImageJ National Institutes of Health N/A Cell Metabolism 37, 1–13.e1–e5, February 4, 2025 e2 mice were housed and fed in a pathogen-free environment at the Laboratory Animal Center of Xi’an Jiaotong University.

    Enzyme-linked Immunosorbent Assay:

    Article Title: Itaconate transporter SLC13A3 confers immunotherapy resistance via alkylation-mediated stabilization of PD-L1.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Fetal bovine serum Biological Industries Cat# 04-001-1ACS NAA Med Chem Express Cat# HY-118628 4-Octyl itaconate Med Chem Express Cat# HY-112675 Opti-MEM Gibco Cat# 31985-062 TRIzol Thermo Fisher Scientific Cat# 15596018 Cycloheximide (CHX) Aladdin Cat#C112766; CAS No. 66-81-9 Critical commercial assays BCA Protein Assay Kit Thermo Fisher Scientific Cat# 23225 PrimeScript RT Master Mix kit Takara Cat# RR036A Mouse IL-1b ELISA kit BOSTER Biological Technology Cat# EK0392 Mouse TNF-a ELISA kit BOSTER Biological Technology Cat# EK0527 Deposited data Raw RNAseq data This paper GSE280490 Data used to generate figures This paper Data S1 Raw data of WB This paper, Mendeley Data https://doi.org/10.17632/k6395ns5pk.1 Experimental models: Cell lines Human: HEK293T ATCC Cat# CRL-11268; RRID: CVCL_1926 Human: THP-1 ATCC Cat# TIB-202; RRID: CVCL_0006 Human: 786-O ATCC Cat# CRL-1932; RRID: CVCL_1051 Human: SW839 ATCC Cat#HTB-49;RRID: CVCL_3604 Human: A549 ATCC Cat#CCL-185;RRID: CVCL_0023 Mouse: Lewis ATCC Cat# CRL-1642; RRID: CVCL_4358 Mouse: Renca ATCC Cat# CRL-2947; RRID: CVCL_2174 Renca Slc13a3 KO cells This paper N/A Experimental models: Organisms/strains Mouse: C57BL/6J Xi’an Jiaotong University N/A Mouse: Slc13a3 KO C57BL/6J Cyagen Biosciences Inc N/A Mouse: Balb/c Xi’an Jiaotong University N/A Oligonucleotides Primers for qPCR analysis, see Table S1 This paper N/A Recombinant DNA pCMV-PD-L1-HA Addgene Cat# 121492 pCMV-8xHis-Ub Addgene Cat# 107392 pCMV-PD-L1 C272A-HA This paper N/A pMD2.G Addgene Cat# 12259 psPAX2 Addgene Cat# 12260 pCMV-PD-L1 C-terminal-GST This paper N/A pCMV-PD-L1 C-terminal C272A-GST This paper N/A Software and algorithms GraphPad Prism 8.0.2 Graphpad N/A FlowJo X software V10.0.07 FlowJo N/A ImageJ National Institutes of Health N/A Cell Metabolism 37, 1–13.e1–e5, February 4, 2025 e2 mice were housed and fed in a pathogen-free environment at the Laboratory Animal Center of Xi’an Jiaotong University. .. REAGENT or RESOURCE SOURCE IDENTIFIER Fetal bovine serum Biological Industries Cat# 04-001-1ACS NAA Med Chem Express Cat# HY-118628 4-Octyl itaconate Med Chem Express Cat# HY-112675 Opti-MEM Gibco Cat# 31985-062 TRIzol Thermo Fisher Scientific Cat# 15596018 Cycloheximide (CHX) Aladdin Cat#C112766; CAS No. 66-81-9 Critical commercial assays BCA Protein Assay Kit Thermo Fisher Scientific Cat# 23225 PrimeScript RT Master Mix kit Takara Cat# RR036A Mouse IL-1b ELISA kit BOSTER Biological Technology Cat# EK0392 Mouse TNF-a ELISA kit BOSTER Biological Technology Cat# EK0527 Deposited data Raw RNAseq data This paper GSE280490 Data used to generate figures This paper Data S1 Raw data of WB This paper, Mendeley Data https://doi.org/10.17632/k6395ns5pk.1 Experimental models: Cell lines Human: HEK293T ATCC Cat# CRL-11268; RRID: CVCL_1926 Human: THP-1 ATCC Cat# TIB-202; RRID: CVCL_0006 Human: 786-O ATCC Cat# CRL-1932; RRID: CVCL_1051 Human: SW839 ATCC Cat#HTB-49;RRID: CVCL_3604 Human: A549 ATCC Cat#CCL-185;RRID: CVCL_0023 Mouse: Lewis ATCC Cat# CRL-1642; RRID: CVCL_4358 Mouse: Renca ATCC Cat# CRL-2947; RRID: CVCL_2174 Renca Slc13a3 KO cells This paper N/A Experimental models: Organisms/strains Mouse: C57BL/6J Xi’an Jiaotong University N/A Mouse: Slc13a3 KO C57BL/6J Cyagen Biosciences Inc N/A Mouse: Balb/c Xi’an Jiaotong University N/A Oligonucleotides Primers for qPCR analysis, see Table S1 This paper N/A Recombinant DNA pCMV-PD-L1-HA Addgene Cat# 121492 pCMV-8xHis-Ub Addgene Cat# 107392 pCMV-PD-L1 C272A-HA This paper N/A pMD2.G Addgene Cat# 12259 psPAX2 Addgene Cat# 12260 pCMV-PD-L1 C-terminal-GST This paper N/A pCMV-PD-L1 C-terminal C272A-GST This paper N/A Software and algorithms GraphPad Prism 8.0.2 Graphpad N/A FlowJo X software V10.0.07 FlowJo N/A ImageJ National Institutes of Health N/A Cell Metabolism 37, 1–13.e1–e5, February 4, 2025 e2 mice were housed and fed in a pathogen-free environment at the Laboratory Animal Center of Xi’an Jiaotong University.

    RNA sequencing:

    Article Title: Itaconate transporter SLC13A3 confers immunotherapy resistance via alkylation-mediated stabilization of PD-L1.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Fetal bovine serum Biological Industries Cat# 04-001-1ACS NAA Med Chem Express Cat# HY-118628 4-Octyl itaconate Med Chem Express Cat# HY-112675 Opti-MEM Gibco Cat# 31985-062 TRIzol Thermo Fisher Scientific Cat# 15596018 Cycloheximide (CHX) Aladdin Cat#C112766; CAS No. 66-81-9 Critical commercial assays BCA Protein Assay Kit Thermo Fisher Scientific Cat# 23225 PrimeScript RT Master Mix kit Takara Cat# RR036A Mouse IL-1b ELISA kit BOSTER Biological Technology Cat# EK0392 Mouse TNF-a ELISA kit BOSTER Biological Technology Cat# EK0527 Deposited data Raw RNAseq data This paper GSE280490 Data used to generate figures This paper Data S1 Raw data of WB This paper, Mendeley Data https://doi.org/10.17632/k6395ns5pk.1 Experimental models: Cell lines Human: HEK293T ATCC Cat# CRL-11268; RRID: CVCL_1926 Human: THP-1 ATCC Cat# TIB-202; RRID: CVCL_0006 Human: 786-O ATCC Cat# CRL-1932; RRID: CVCL_1051 Human: SW839 ATCC Cat#HTB-49;RRID: CVCL_3604 Human: A549 ATCC Cat#CCL-185;RRID: CVCL_0023 Mouse: Lewis ATCC Cat# CRL-1642; RRID: CVCL_4358 Mouse: Renca ATCC Cat# CRL-2947; RRID: CVCL_2174 Renca Slc13a3 KO cells This paper N/A Experimental models: Organisms/strains Mouse: C57BL/6J Xi’an Jiaotong University N/A Mouse: Slc13a3 KO C57BL/6J Cyagen Biosciences Inc N/A Mouse: Balb/c Xi’an Jiaotong University N/A Oligonucleotides Primers for qPCR analysis, see Table S1 This paper N/A Recombinant DNA pCMV-PD-L1-HA Addgene Cat# 121492 pCMV-8xHis-Ub Addgene Cat# 107392 pCMV-PD-L1 C272A-HA This paper N/A pMD2.G Addgene Cat# 12259 psPAX2 Addgene Cat# 12260 pCMV-PD-L1 C-terminal-GST This paper N/A pCMV-PD-L1 C-terminal C272A-GST This paper N/A Software and algorithms GraphPad Prism 8.0.2 Graphpad N/A FlowJo X software V10.0.07 FlowJo N/A ImageJ National Institutes of Health N/A Cell Metabolism 37, 1–13.e1–e5, February 4, 2025 e2 mice were housed and fed in a pathogen-free environment at the Laboratory Animal Center of Xi’an Jiaotong University. .. REAGENT or RESOURCE SOURCE IDENTIFIER Fetal bovine serum Biological Industries Cat# 04-001-1ACS NAA Med Chem Express Cat# HY-118628 4-Octyl itaconate Med Chem Express Cat# HY-112675 Opti-MEM Gibco Cat# 31985-062 TRIzol Thermo Fisher Scientific Cat# 15596018 Cycloheximide (CHX) Aladdin Cat#C112766; CAS No. 66-81-9 Critical commercial assays BCA Protein Assay Kit Thermo Fisher Scientific Cat# 23225 PrimeScript RT Master Mix kit Takara Cat# RR036A Mouse IL-1b ELISA kit BOSTER Biological Technology Cat# EK0392 Mouse TNF-a ELISA kit BOSTER Biological Technology Cat# EK0527 Deposited data Raw RNAseq data This paper GSE280490 Data used to generate figures This paper Data S1 Raw data of WB This paper, Mendeley Data https://doi.org/10.17632/k6395ns5pk.1 Experimental models: Cell lines Human: HEK293T ATCC Cat# CRL-11268; RRID: CVCL_1926 Human: THP-1 ATCC Cat# TIB-202; RRID: CVCL_0006 Human: 786-O ATCC Cat# CRL-1932; RRID: CVCL_1051 Human: SW839 ATCC Cat#HTB-49;RRID: CVCL_3604 Human: A549 ATCC Cat#CCL-185;RRID: CVCL_0023 Mouse: Lewis ATCC Cat# CRL-1642; RRID: CVCL_4358 Mouse: Renca ATCC Cat# CRL-2947; RRID: CVCL_2174 Renca Slc13a3 KO cells This paper N/A Experimental models: Organisms/strains Mouse: C57BL/6J Xi’an Jiaotong University N/A Mouse: Slc13a3 KO C57BL/6J Cyagen Biosciences Inc N/A Mouse: Balb/c Xi’an Jiaotong University N/A Oligonucleotides Primers for qPCR analysis, see Table S1 This paper N/A Recombinant DNA pCMV-PD-L1-HA Addgene Cat# 121492 pCMV-8xHis-Ub Addgene Cat# 107392 pCMV-PD-L1 C272A-HA This paper N/A pMD2.G Addgene Cat# 12259 psPAX2 Addgene Cat# 12260 pCMV-PD-L1 C-terminal-GST This paper N/A pCMV-PD-L1 C-terminal C272A-GST This paper N/A Software and algorithms GraphPad Prism 8.0.2 Graphpad N/A FlowJo X software V10.0.07 FlowJo N/A ImageJ National Institutes of Health N/A Cell Metabolism 37, 1–13.e1–e5, February 4, 2025 e2 mice were housed and fed in a pathogen-free environment at the Laboratory Animal Center of Xi’an Jiaotong University.

    Western Blot:

    Article Title: Itaconate transporter SLC13A3 confers immunotherapy resistance via alkylation-mediated stabilization of PD-L1.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Fetal bovine serum Biological Industries Cat# 04-001-1ACS NAA Med Chem Express Cat# HY-118628 4-Octyl itaconate Med Chem Express Cat# HY-112675 Opti-MEM Gibco Cat# 31985-062 TRIzol Thermo Fisher Scientific Cat# 15596018 Cycloheximide (CHX) Aladdin Cat#C112766; CAS No. 66-81-9 Critical commercial assays BCA Protein Assay Kit Thermo Fisher Scientific Cat# 23225 PrimeScript RT Master Mix kit Takara Cat# RR036A Mouse IL-1b ELISA kit BOSTER Biological Technology Cat# EK0392 Mouse TNF-a ELISA kit BOSTER Biological Technology Cat# EK0527 Deposited data Raw RNAseq data This paper GSE280490 Data used to generate figures This paper Data S1 Raw data of WB This paper, Mendeley Data https://doi.org/10.17632/k6395ns5pk.1 Experimental models: Cell lines Human: HEK293T ATCC Cat# CRL-11268; RRID: CVCL_1926 Human: THP-1 ATCC Cat# TIB-202; RRID: CVCL_0006 Human: 786-O ATCC Cat# CRL-1932; RRID: CVCL_1051 Human: SW839 ATCC Cat#HTB-49;RRID: CVCL_3604 Human: A549 ATCC Cat#CCL-185;RRID: CVCL_0023 Mouse: Lewis ATCC Cat# CRL-1642; RRID: CVCL_4358 Mouse: Renca ATCC Cat# CRL-2947; RRID: CVCL_2174 Renca Slc13a3 KO cells This paper N/A Experimental models: Organisms/strains Mouse: C57BL/6J Xi’an Jiaotong University N/A Mouse: Slc13a3 KO C57BL/6J Cyagen Biosciences Inc N/A Mouse: Balb/c Xi’an Jiaotong University N/A Oligonucleotides Primers for qPCR analysis, see Table S1 This paper N/A Recombinant DNA pCMV-PD-L1-HA Addgene Cat# 121492 pCMV-8xHis-Ub Addgene Cat# 107392 pCMV-PD-L1 C272A-HA This paper N/A pMD2.G Addgene Cat# 12259 psPAX2 Addgene Cat# 12260 pCMV-PD-L1 C-terminal-GST This paper N/A pCMV-PD-L1 C-terminal C272A-GST This paper N/A Software and algorithms GraphPad Prism 8.0.2 Graphpad N/A FlowJo X software V10.0.07 FlowJo N/A ImageJ National Institutes of Health N/A Cell Metabolism 37, 1–13.e1–e5, February 4, 2025 e2 mice were housed and fed in a pathogen-free environment at the Laboratory Animal Center of Xi’an Jiaotong University. .. REAGENT or RESOURCE SOURCE IDENTIFIER Fetal bovine serum Biological Industries Cat# 04-001-1ACS NAA Med Chem Express Cat# HY-118628 4-Octyl itaconate Med Chem Express Cat# HY-112675 Opti-MEM Gibco Cat# 31985-062 TRIzol Thermo Fisher Scientific Cat# 15596018 Cycloheximide (CHX) Aladdin Cat#C112766; CAS No. 66-81-9 Critical commercial assays BCA Protein Assay Kit Thermo Fisher Scientific Cat# 23225 PrimeScript RT Master Mix kit Takara Cat# RR036A Mouse IL-1b ELISA kit BOSTER Biological Technology Cat# EK0392 Mouse TNF-a ELISA kit BOSTER Biological Technology Cat# EK0527 Deposited data Raw RNAseq data This paper GSE280490 Data used to generate figures This paper Data S1 Raw data of WB This paper, Mendeley Data https://doi.org/10.17632/k6395ns5pk.1 Experimental models: Cell lines Human: HEK293T ATCC Cat# CRL-11268; RRID: CVCL_1926 Human: THP-1 ATCC Cat# TIB-202; RRID: CVCL_0006 Human: 786-O ATCC Cat# CRL-1932; RRID: CVCL_1051 Human: SW839 ATCC Cat#HTB-49;RRID: CVCL_3604 Human: A549 ATCC Cat#CCL-185;RRID: CVCL_0023 Mouse: Lewis ATCC Cat# CRL-1642; RRID: CVCL_4358 Mouse: Renca ATCC Cat# CRL-2947; RRID: CVCL_2174 Renca Slc13a3 KO cells This paper N/A Experimental models: Organisms/strains Mouse: C57BL/6J Xi’an Jiaotong University N/A Mouse: Slc13a3 KO C57BL/6J Cyagen Biosciences Inc N/A Mouse: Balb/c Xi’an Jiaotong University N/A Oligonucleotides Primers for qPCR analysis, see Table S1 This paper N/A Recombinant DNA pCMV-PD-L1-HA Addgene Cat# 121492 pCMV-8xHis-Ub Addgene Cat# 107392 pCMV-PD-L1 C272A-HA This paper N/A pMD2.G Addgene Cat# 12259 psPAX2 Addgene Cat# 12260 pCMV-PD-L1 C-terminal-GST This paper N/A pCMV-PD-L1 C-terminal C272A-GST This paper N/A Software and algorithms GraphPad Prism 8.0.2 Graphpad N/A FlowJo X software V10.0.07 FlowJo N/A ImageJ National Institutes of Health N/A Cell Metabolism 37, 1–13.e1–e5, February 4, 2025 e2 mice were housed and fed in a pathogen-free environment at the Laboratory Animal Center of Xi’an Jiaotong University.

    Real-time Polymerase Chain Reaction:

    Article Title: Itaconate transporter SLC13A3 confers immunotherapy resistance via alkylation-mediated stabilization of PD-L1.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Fetal bovine serum Biological Industries Cat# 04-001-1ACS NAA Med Chem Express Cat# HY-118628 4-Octyl itaconate Med Chem Express Cat# HY-112675 Opti-MEM Gibco Cat# 31985-062 TRIzol Thermo Fisher Scientific Cat# 15596018 Cycloheximide (CHX) Aladdin Cat#C112766; CAS No. 66-81-9 Critical commercial assays BCA Protein Assay Kit Thermo Fisher Scientific Cat# 23225 PrimeScript RT Master Mix kit Takara Cat# RR036A Mouse IL-1b ELISA kit BOSTER Biological Technology Cat# EK0392 Mouse TNF-a ELISA kit BOSTER Biological Technology Cat# EK0527 Deposited data Raw RNAseq data This paper GSE280490 Data used to generate figures This paper Data S1 Raw data of WB This paper, Mendeley Data https://doi.org/10.17632/k6395ns5pk.1 Experimental models: Cell lines Human: HEK293T ATCC Cat# CRL-11268; RRID: CVCL_1926 Human: THP-1 ATCC Cat# TIB-202; RRID: CVCL_0006 Human: 786-O ATCC Cat# CRL-1932; RRID: CVCL_1051 Human: SW839 ATCC Cat#HTB-49;RRID: CVCL_3604 Human: A549 ATCC Cat#CCL-185;RRID: CVCL_0023 Mouse: Lewis ATCC Cat# CRL-1642; RRID: CVCL_4358 Mouse: Renca ATCC Cat# CRL-2947; RRID: CVCL_2174 Renca Slc13a3 KO cells This paper N/A Experimental models: Organisms/strains Mouse: C57BL/6J Xi’an Jiaotong University N/A Mouse: Slc13a3 KO C57BL/6J Cyagen Biosciences Inc N/A Mouse: Balb/c Xi’an Jiaotong University N/A Oligonucleotides Primers for qPCR analysis, see Table S1 This paper N/A Recombinant DNA pCMV-PD-L1-HA Addgene Cat# 121492 pCMV-8xHis-Ub Addgene Cat# 107392 pCMV-PD-L1 C272A-HA This paper N/A pMD2.G Addgene Cat# 12259 psPAX2 Addgene Cat# 12260 pCMV-PD-L1 C-terminal-GST This paper N/A pCMV-PD-L1 C-terminal C272A-GST This paper N/A Software and algorithms GraphPad Prism 8.0.2 Graphpad N/A FlowJo X software V10.0.07 FlowJo N/A ImageJ National Institutes of Health N/A Cell Metabolism 37, 1–13.e1–e5, February 4, 2025 e2 mice were housed and fed in a pathogen-free environment at the Laboratory Animal Center of Xi’an Jiaotong University. .. REAGENT or RESOURCE SOURCE IDENTIFIER Fetal bovine serum Biological Industries Cat# 04-001-1ACS NAA Med Chem Express Cat# HY-118628 4-Octyl itaconate Med Chem Express Cat# HY-112675 Opti-MEM Gibco Cat# 31985-062 TRIzol Thermo Fisher Scientific Cat# 15596018 Cycloheximide (CHX) Aladdin Cat#C112766; CAS No. 66-81-9 Critical commercial assays BCA Protein Assay Kit Thermo Fisher Scientific Cat# 23225 PrimeScript RT Master Mix kit Takara Cat# RR036A Mouse IL-1b ELISA kit BOSTER Biological Technology Cat# EK0392 Mouse TNF-a ELISA kit BOSTER Biological Technology Cat# EK0527 Deposited data Raw RNAseq data This paper GSE280490 Data used to generate figures This paper Data S1 Raw data of WB This paper, Mendeley Data https://doi.org/10.17632/k6395ns5pk.1 Experimental models: Cell lines Human: HEK293T ATCC Cat# CRL-11268; RRID: CVCL_1926 Human: THP-1 ATCC Cat# TIB-202; RRID: CVCL_0006 Human: 786-O ATCC Cat# CRL-1932; RRID: CVCL_1051 Human: SW839 ATCC Cat#HTB-49;RRID: CVCL_3604 Human: A549 ATCC Cat#CCL-185;RRID: CVCL_0023 Mouse: Lewis ATCC Cat# CRL-1642; RRID: CVCL_4358 Mouse: Renca ATCC Cat# CRL-2947; RRID: CVCL_2174 Renca Slc13a3 KO cells This paper N/A Experimental models: Organisms/strains Mouse: C57BL/6J Xi’an Jiaotong University N/A Mouse: Slc13a3 KO C57BL/6J Cyagen Biosciences Inc N/A Mouse: Balb/c Xi’an Jiaotong University N/A Oligonucleotides Primers for qPCR analysis, see Table S1 This paper N/A Recombinant DNA pCMV-PD-L1-HA Addgene Cat# 121492 pCMV-8xHis-Ub Addgene Cat# 107392 pCMV-PD-L1 C272A-HA This paper N/A pMD2.G Addgene Cat# 12259 psPAX2 Addgene Cat# 12260 pCMV-PD-L1 C-terminal-GST This paper N/A pCMV-PD-L1 C-terminal C272A-GST This paper N/A Software and algorithms GraphPad Prism 8.0.2 Graphpad N/A FlowJo X software V10.0.07 FlowJo N/A ImageJ National Institutes of Health N/A Cell Metabolism 37, 1–13.e1–e5, February 4, 2025 e2 mice were housed and fed in a pathogen-free environment at the Laboratory Animal Center of Xi’an Jiaotong University.

    Recombinant:

    Article Title: Itaconate transporter SLC13A3 confers immunotherapy resistance via alkylation-mediated stabilization of PD-L1.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Fetal bovine serum Biological Industries Cat# 04-001-1ACS NAA Med Chem Express Cat# HY-118628 4-Octyl itaconate Med Chem Express Cat# HY-112675 Opti-MEM Gibco Cat# 31985-062 TRIzol Thermo Fisher Scientific Cat# 15596018 Cycloheximide (CHX) Aladdin Cat#C112766; CAS No. 66-81-9 Critical commercial assays BCA Protein Assay Kit Thermo Fisher Scientific Cat# 23225 PrimeScript RT Master Mix kit Takara Cat# RR036A Mouse IL-1b ELISA kit BOSTER Biological Technology Cat# EK0392 Mouse TNF-a ELISA kit BOSTER Biological Technology Cat# EK0527 Deposited data Raw RNAseq data This paper GSE280490 Data used to generate figures This paper Data S1 Raw data of WB This paper, Mendeley Data https://doi.org/10.17632/k6395ns5pk.1 Experimental models: Cell lines Human: HEK293T ATCC Cat# CRL-11268; RRID: CVCL_1926 Human: THP-1 ATCC Cat# TIB-202; RRID: CVCL_0006 Human: 786-O ATCC Cat# CRL-1932; RRID: CVCL_1051 Human: SW839 ATCC Cat#HTB-49;RRID: CVCL_3604 Human: A549 ATCC Cat#CCL-185;RRID: CVCL_0023 Mouse: Lewis ATCC Cat# CRL-1642; RRID: CVCL_4358 Mouse: Renca ATCC Cat# CRL-2947; RRID: CVCL_2174 Renca Slc13a3 KO cells This paper N/A Experimental models: Organisms/strains Mouse: C57BL/6J Xi’an Jiaotong University N/A Mouse: Slc13a3 KO C57BL/6J Cyagen Biosciences Inc N/A Mouse: Balb/c Xi’an Jiaotong University N/A Oligonucleotides Primers for qPCR analysis, see Table S1 This paper N/A Recombinant DNA pCMV-PD-L1-HA Addgene Cat# 121492 pCMV-8xHis-Ub Addgene Cat# 107392 pCMV-PD-L1 C272A-HA This paper N/A pMD2.G Addgene Cat# 12259 psPAX2 Addgene Cat# 12260 pCMV-PD-L1 C-terminal-GST This paper N/A pCMV-PD-L1 C-terminal C272A-GST This paper N/A Software and algorithms GraphPad Prism 8.0.2 Graphpad N/A FlowJo X software V10.0.07 FlowJo N/A ImageJ National Institutes of Health N/A Cell Metabolism 37, 1–13.e1–e5, February 4, 2025 e2 mice were housed and fed in a pathogen-free environment at the Laboratory Animal Center of Xi’an Jiaotong University. .. REAGENT or RESOURCE SOURCE IDENTIFIER Fetal bovine serum Biological Industries Cat# 04-001-1ACS NAA Med Chem Express Cat# HY-118628 4-Octyl itaconate Med Chem Express Cat# HY-112675 Opti-MEM Gibco Cat# 31985-062 TRIzol Thermo Fisher Scientific Cat# 15596018 Cycloheximide (CHX) Aladdin Cat#C112766; CAS No. 66-81-9 Critical commercial assays BCA Protein Assay Kit Thermo Fisher Scientific Cat# 23225 PrimeScript RT Master Mix kit Takara Cat# RR036A Mouse IL-1b ELISA kit BOSTER Biological Technology Cat# EK0392 Mouse TNF-a ELISA kit BOSTER Biological Technology Cat# EK0527 Deposited data Raw RNAseq data This paper GSE280490 Data used to generate figures This paper Data S1 Raw data of WB This paper, Mendeley Data https://doi.org/10.17632/k6395ns5pk.1 Experimental models: Cell lines Human: HEK293T ATCC Cat# CRL-11268; RRID: CVCL_1926 Human: THP-1 ATCC Cat# TIB-202; RRID: CVCL_0006 Human: 786-O ATCC Cat# CRL-1932; RRID: CVCL_1051 Human: SW839 ATCC Cat#HTB-49;RRID: CVCL_3604 Human: A549 ATCC Cat#CCL-185;RRID: CVCL_0023 Mouse: Lewis ATCC Cat# CRL-1642; RRID: CVCL_4358 Mouse: Renca ATCC Cat# CRL-2947; RRID: CVCL_2174 Renca Slc13a3 KO cells This paper N/A Experimental models: Organisms/strains Mouse: C57BL/6J Xi’an Jiaotong University N/A Mouse: Slc13a3 KO C57BL/6J Cyagen Biosciences Inc N/A Mouse: Balb/c Xi’an Jiaotong University N/A Oligonucleotides Primers for qPCR analysis, see Table S1 This paper N/A Recombinant DNA pCMV-PD-L1-HA Addgene Cat# 121492 pCMV-8xHis-Ub Addgene Cat# 107392 pCMV-PD-L1 C272A-HA This paper N/A pMD2.G Addgene Cat# 12259 psPAX2 Addgene Cat# 12260 pCMV-PD-L1 C-terminal-GST This paper N/A pCMV-PD-L1 C-terminal C272A-GST This paper N/A Software and algorithms GraphPad Prism 8.0.2 Graphpad N/A FlowJo X software V10.0.07 FlowJo N/A ImageJ National Institutes of Health N/A Cell Metabolism 37, 1–13.e1–e5, February 4, 2025 e2 mice were housed and fed in a pathogen-free environment at the Laboratory Animal Center of Xi’an Jiaotong University.

    Software:

    Article Title: Itaconate transporter SLC13A3 confers immunotherapy resistance via alkylation-mediated stabilization of PD-L1.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Fetal bovine serum Biological Industries Cat# 04-001-1ACS NAA Med Chem Express Cat# HY-118628 4-Octyl itaconate Med Chem Express Cat# HY-112675 Opti-MEM Gibco Cat# 31985-062 TRIzol Thermo Fisher Scientific Cat# 15596018 Cycloheximide (CHX) Aladdin Cat#C112766; CAS No. 66-81-9 Critical commercial assays BCA Protein Assay Kit Thermo Fisher Scientific Cat# 23225 PrimeScript RT Master Mix kit Takara Cat# RR036A Mouse IL-1b ELISA kit BOSTER Biological Technology Cat# EK0392 Mouse TNF-a ELISA kit BOSTER Biological Technology Cat# EK0527 Deposited data Raw RNAseq data This paper GSE280490 Data used to generate figures This paper Data S1 Raw data of WB This paper, Mendeley Data https://doi.org/10.17632/k6395ns5pk.1 Experimental models: Cell lines Human: HEK293T ATCC Cat# CRL-11268; RRID: CVCL_1926 Human: THP-1 ATCC Cat# TIB-202; RRID: CVCL_0006 Human: 786-O ATCC Cat# CRL-1932; RRID: CVCL_1051 Human: SW839 ATCC Cat#HTB-49;RRID: CVCL_3604 Human: A549 ATCC Cat#CCL-185;RRID: CVCL_0023 Mouse: Lewis ATCC Cat# CRL-1642; RRID: CVCL_4358 Mouse: Renca ATCC Cat# CRL-2947; RRID: CVCL_2174 Renca Slc13a3 KO cells This paper N/A Experimental models: Organisms/strains Mouse: C57BL/6J Xi’an Jiaotong University N/A Mouse: Slc13a3 KO C57BL/6J Cyagen Biosciences Inc N/A Mouse: Balb/c Xi’an Jiaotong University N/A Oligonucleotides Primers for qPCR analysis, see Table S1 This paper N/A Recombinant DNA pCMV-PD-L1-HA Addgene Cat# 121492 pCMV-8xHis-Ub Addgene Cat# 107392 pCMV-PD-L1 C272A-HA This paper N/A pMD2.G Addgene Cat# 12259 psPAX2 Addgene Cat# 12260 pCMV-PD-L1 C-terminal-GST This paper N/A pCMV-PD-L1 C-terminal C272A-GST This paper N/A Software and algorithms GraphPad Prism 8.0.2 Graphpad N/A FlowJo X software V10.0.07 FlowJo N/A ImageJ National Institutes of Health N/A Cell Metabolism 37, 1–13.e1–e5, February 4, 2025 e2 mice were housed and fed in a pathogen-free environment at the Laboratory Animal Center of Xi’an Jiaotong University. .. REAGENT or RESOURCE SOURCE IDENTIFIER Fetal bovine serum Biological Industries Cat# 04-001-1ACS NAA Med Chem Express Cat# HY-118628 4-Octyl itaconate Med Chem Express Cat# HY-112675 Opti-MEM Gibco Cat# 31985-062 TRIzol Thermo Fisher Scientific Cat# 15596018 Cycloheximide (CHX) Aladdin Cat#C112766; CAS No. 66-81-9 Critical commercial assays BCA Protein Assay Kit Thermo Fisher Scientific Cat# 23225 PrimeScript RT Master Mix kit Takara Cat# RR036A Mouse IL-1b ELISA kit BOSTER Biological Technology Cat# EK0392 Mouse TNF-a ELISA kit BOSTER Biological Technology Cat# EK0527 Deposited data Raw RNAseq data This paper GSE280490 Data used to generate figures This paper Data S1 Raw data of WB This paper, Mendeley Data https://doi.org/10.17632/k6395ns5pk.1 Experimental models: Cell lines Human: HEK293T ATCC Cat# CRL-11268; RRID: CVCL_1926 Human: THP-1 ATCC Cat# TIB-202; RRID: CVCL_0006 Human: 786-O ATCC Cat# CRL-1932; RRID: CVCL_1051 Human: SW839 ATCC Cat#HTB-49;RRID: CVCL_3604 Human: A549 ATCC Cat#CCL-185;RRID: CVCL_0023 Mouse: Lewis ATCC Cat# CRL-1642; RRID: CVCL_4358 Mouse: Renca ATCC Cat# CRL-2947; RRID: CVCL_2174 Renca Slc13a3 KO cells This paper N/A Experimental models: Organisms/strains Mouse: C57BL/6J Xi’an Jiaotong University N/A Mouse: Slc13a3 KO C57BL/6J Cyagen Biosciences Inc N/A Mouse: Balb/c Xi’an Jiaotong University N/A Oligonucleotides Primers for qPCR analysis, see Table S1 This paper N/A Recombinant DNA pCMV-PD-L1-HA Addgene Cat# 121492 pCMV-8xHis-Ub Addgene Cat# 107392 pCMV-PD-L1 C272A-HA This paper N/A pMD2.G Addgene Cat# 12259 psPAX2 Addgene Cat# 12260 pCMV-PD-L1 C-terminal-GST This paper N/A pCMV-PD-L1 C-terminal C272A-GST This paper N/A Software and algorithms GraphPad Prism 8.0.2 Graphpad N/A FlowJo X software V10.0.07 FlowJo N/A ImageJ National Institutes of Health N/A Cell Metabolism 37, 1–13.e1–e5, February 4, 2025 e2 mice were housed and fed in a pathogen-free environment at the Laboratory Animal Center of Xi’an Jiaotong University.

    other:

    Article Title: Bivalent inhibitors of the BTB E3 ligase KEAP1 enable instant NRF2 activation to suppress acute inflammatory response.
    Article Snippet: Article Bivalent inhibitors of the BTB E3 ligase KEAP1 enable instant NRF2 activation to suppress acute inflammatory response



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    Figure 2. Contralateral <t>LCN2</t> elevation arises from local astrocytes and trans-callosal spread (A and B) Representative images and quantifications showing time-course changes of astrocytic LCN2 in the contralateral corpus callosum (n = 5 mice; scale bar, 20 μm). (C and D) Immunoblotting and quantitative analysis of LCN2 (n = 5 mice).
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    Image Search Results


    Figure 2. Contralateral LCN2 elevation arises from local astrocytes and trans-callosal spread (A and B) Representative images and quantifications showing time-course changes of astrocytic LCN2 in the contralateral corpus callosum (n = 5 mice; scale bar, 20 μm). (C and D) Immunoblotting and quantitative analysis of LCN2 (n = 5 mice).

    Journal: Cell reports

    Article Title: Lipocalin-2 regulates astrocyte-oligodendrocyte interaction to drive post-stroke secondary demyelination.

    doi: 10.1016/j.celrep.2025.115899

    Figure Lengend Snippet: Figure 2. Contralateral LCN2 elevation arises from local astrocytes and trans-callosal spread (A and B) Representative images and quantifications showing time-course changes of astrocytic LCN2 in the contralateral corpus callosum (n = 5 mice; scale bar, 20 μm). (C and D) Immunoblotting and quantitative analysis of LCN2 (n = 5 mice).

    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Alex Fluor 647 affiniPure Donkey Anti-Goat IgG(H + L) Jackson Cat# 705-605-003; RRID: AB_2340436 Alex Fluor 488 affiniPure Donkey Anti-Rabbit IgG(H + L) Jackson Cat# 711-545-152; RRID: AB_2313584 Alex Fluor 594 affiniPure Donkey Anti-Rabbit IgG(H + L) Jackson Cat# 711-585-152; RRID: AB_2340621 Alex Fluor 647 affiniPure Donkey Anti-Rabbit IgG(H + L) Jackson Cat# 711-605-152; RRID: AB_2492288 Alex Fluor 488 affiniPure Donkey Anti-Mouse IgG(H + L) Jackson Cat# 715-545-150; RRID: AB_2340846 Alex Fluor 594 affiniPure Donkey Anti-Mouse IgG(H + L) Jackson Cat# 715-585-150; RRID: AB_2340854 Alex Fluor 647 affiniPure Donkey Anti-Mouse IgG(H + L) Jackson Cat# 715-605-150; RRID: AB_2340862 Alex Fluor 488affiniPure Donkey Anti-Rat IgG(H + L) Jackson Cat# 712-545-150; RRID: AB_2340683 Alex Fluor 594 affiniPure Donkey Anti-Rat IgG(H + L) Jackson Cat# 712-585-150; RRID: AB_2340688 Alex Fluor 647 affiniPure Donkey Anti-Rat IgG(H + L) Jackson Cat# 712-605-150; RRID: AB_2340693 Anti-Mouse IgG, HRP-linked antibody Cell Signaling Technology Cat# 7076S; RRID: AB_330924 Anti-Rabbit IgG, HRP-linked antibody Cell Signaling Technology Cat# 7074S; RRID: AB_2099233 Anti-Goat IgG, HRP-linked antibody Santa Cruz Cat# sc-2354; RRID: AB_628490 Anti-Mouse IgG for IP, HRP-linked antibody Abcam Cat# ab131368; RRID: AB_2895114 Anti-Goat IgG for IP, HRP-linked antibody Abcam Cat# ab157532; RRID: AB_3676695 Bacterial and virus strains AAV-hLCN2 GeneChem N/A AAV-Gfap-Cre GeneChem N/A AAV-Lcn2 GeneChem N/A Lrp2-shRNA GeneChem N/A Perk-shRNA GeneChem N/A AAV-Lcn2(Δ2-20) GeneChem N/A LV-Lcn2(Δ2-20) GeneChem N/A LV-Lcn2 GeneChem N/A Chemicals, peptides, and recombinant proteins TRIzol Sigma Cat# T9424 DMEM/F12 Gibco Cat# 2749772 Poly-D-Lysine sigma Cat# P6407 L-glutamine Gibco Cat# 35050061 Penicillin-streptomycin Gibco Cat# 15140122 Fetal Bovine Serum Gibco Cat# A5669701 LPS Sigma Cat# L4391-1MG ITSS Roche Cat# 11074547001 PDGF-BB Biolegend Cat# 558804 b-FGF Biolegend Cat# 579604 BSA Sigma Cat# A1193 CNTF PROSPEC Cat# CYT-139 Protease inhibitor cocktail Beyotime Cat# P1006 protein A/G agarose Santa Cruz Cat# sc-2003 CCK-8 kit Beyotime Cat# C0037 LCN2 Elisa kit Boster biological technology Cat# EK0854 (Continued on next page) 20 Cell Reports 44, 115899, July 22, 2025

    Techniques: Western Blot

    Figure 4. Extracellular LCN2 gradually accumulates within mature oligodendrocytes (A and B) Representative images and quantifications showing LCN2 (red) surrounded by and accumulated into mature oligodendrocytes (GST-pi + , green; Olig2 + , blue) in the contralateral corpus callosum after dMCAO (n = 5 mice; scale bar, 20 μm). (C) Experimental flowchart in vivo. (D and E) Representative images and quantitative analysis of GFP (green) reporter LV into astrocytes (GFAP, blue) with induced hLCN2 (red) in the corpus callosum of Lcn2 − /− mice. White arrows indicate transfected astrocytes (scale bar, 20 μm).

    Journal: Cell reports

    Article Title: Lipocalin-2 regulates astrocyte-oligodendrocyte interaction to drive post-stroke secondary demyelination.

    doi: 10.1016/j.celrep.2025.115899

    Figure Lengend Snippet: Figure 4. Extracellular LCN2 gradually accumulates within mature oligodendrocytes (A and B) Representative images and quantifications showing LCN2 (red) surrounded by and accumulated into mature oligodendrocytes (GST-pi + , green; Olig2 + , blue) in the contralateral corpus callosum after dMCAO (n = 5 mice; scale bar, 20 μm). (C) Experimental flowchart in vivo. (D and E) Representative images and quantitative analysis of GFP (green) reporter LV into astrocytes (GFAP, blue) with induced hLCN2 (red) in the corpus callosum of Lcn2 − /− mice. White arrows indicate transfected astrocytes (scale bar, 20 μm).

    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Alex Fluor 647 affiniPure Donkey Anti-Goat IgG(H + L) Jackson Cat# 705-605-003; RRID: AB_2340436 Alex Fluor 488 affiniPure Donkey Anti-Rabbit IgG(H + L) Jackson Cat# 711-545-152; RRID: AB_2313584 Alex Fluor 594 affiniPure Donkey Anti-Rabbit IgG(H + L) Jackson Cat# 711-585-152; RRID: AB_2340621 Alex Fluor 647 affiniPure Donkey Anti-Rabbit IgG(H + L) Jackson Cat# 711-605-152; RRID: AB_2492288 Alex Fluor 488 affiniPure Donkey Anti-Mouse IgG(H + L) Jackson Cat# 715-545-150; RRID: AB_2340846 Alex Fluor 594 affiniPure Donkey Anti-Mouse IgG(H + L) Jackson Cat# 715-585-150; RRID: AB_2340854 Alex Fluor 647 affiniPure Donkey Anti-Mouse IgG(H + L) Jackson Cat# 715-605-150; RRID: AB_2340862 Alex Fluor 488affiniPure Donkey Anti-Rat IgG(H + L) Jackson Cat# 712-545-150; RRID: AB_2340683 Alex Fluor 594 affiniPure Donkey Anti-Rat IgG(H + L) Jackson Cat# 712-585-150; RRID: AB_2340688 Alex Fluor 647 affiniPure Donkey Anti-Rat IgG(H + L) Jackson Cat# 712-605-150; RRID: AB_2340693 Anti-Mouse IgG, HRP-linked antibody Cell Signaling Technology Cat# 7076S; RRID: AB_330924 Anti-Rabbit IgG, HRP-linked antibody Cell Signaling Technology Cat# 7074S; RRID: AB_2099233 Anti-Goat IgG, HRP-linked antibody Santa Cruz Cat# sc-2354; RRID: AB_628490 Anti-Mouse IgG for IP, HRP-linked antibody Abcam Cat# ab131368; RRID: AB_2895114 Anti-Goat IgG for IP, HRP-linked antibody Abcam Cat# ab157532; RRID: AB_3676695 Bacterial and virus strains AAV-hLCN2 GeneChem N/A AAV-Gfap-Cre GeneChem N/A AAV-Lcn2 GeneChem N/A Lrp2-shRNA GeneChem N/A Perk-shRNA GeneChem N/A AAV-Lcn2(Δ2-20) GeneChem N/A LV-Lcn2(Δ2-20) GeneChem N/A LV-Lcn2 GeneChem N/A Chemicals, peptides, and recombinant proteins TRIzol Sigma Cat# T9424 DMEM/F12 Gibco Cat# 2749772 Poly-D-Lysine sigma Cat# P6407 L-glutamine Gibco Cat# 35050061 Penicillin-streptomycin Gibco Cat# 15140122 Fetal Bovine Serum Gibco Cat# A5669701 LPS Sigma Cat# L4391-1MG ITSS Roche Cat# 11074547001 PDGF-BB Biolegend Cat# 558804 b-FGF Biolegend Cat# 579604 BSA Sigma Cat# A1193 CNTF PROSPEC Cat# CYT-139 Protease inhibitor cocktail Beyotime Cat# P1006 protein A/G agarose Santa Cruz Cat# sc-2003 CCK-8 kit Beyotime Cat# C0037 LCN2 Elisa kit Boster biological technology Cat# EK0854 (Continued on next page) 20 Cell Reports 44, 115899, July 22, 2025

    Techniques: In Vivo, Transfection

    Figure 7. Oligodendrocyte Lrp2 knockdown suppresses LCN2-related white matter degeneration (A) Relative mRNA expression levels of LCN2 receptors (Lrp2, 24p3r, Lrp6, Mc1r, Mc3r, Mc4r) (n = 5 independent primary cell cultures). (B and C) Representative fluorescent images and quantification of LRP2 expression in cultured oligodendrocytes (n = 5 mice; scale bar, 20 μm).

    Journal: Cell reports

    Article Title: Lipocalin-2 regulates astrocyte-oligodendrocyte interaction to drive post-stroke secondary demyelination.

    doi: 10.1016/j.celrep.2025.115899

    Figure Lengend Snippet: Figure 7. Oligodendrocyte Lrp2 knockdown suppresses LCN2-related white matter degeneration (A) Relative mRNA expression levels of LCN2 receptors (Lrp2, 24p3r, Lrp6, Mc1r, Mc3r, Mc4r) (n = 5 independent primary cell cultures). (B and C) Representative fluorescent images and quantification of LRP2 expression in cultured oligodendrocytes (n = 5 mice; scale bar, 20 μm).

    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Alex Fluor 647 affiniPure Donkey Anti-Goat IgG(H + L) Jackson Cat# 705-605-003; RRID: AB_2340436 Alex Fluor 488 affiniPure Donkey Anti-Rabbit IgG(H + L) Jackson Cat# 711-545-152; RRID: AB_2313584 Alex Fluor 594 affiniPure Donkey Anti-Rabbit IgG(H + L) Jackson Cat# 711-585-152; RRID: AB_2340621 Alex Fluor 647 affiniPure Donkey Anti-Rabbit IgG(H + L) Jackson Cat# 711-605-152; RRID: AB_2492288 Alex Fluor 488 affiniPure Donkey Anti-Mouse IgG(H + L) Jackson Cat# 715-545-150; RRID: AB_2340846 Alex Fluor 594 affiniPure Donkey Anti-Mouse IgG(H + L) Jackson Cat# 715-585-150; RRID: AB_2340854 Alex Fluor 647 affiniPure Donkey Anti-Mouse IgG(H + L) Jackson Cat# 715-605-150; RRID: AB_2340862 Alex Fluor 488affiniPure Donkey Anti-Rat IgG(H + L) Jackson Cat# 712-545-150; RRID: AB_2340683 Alex Fluor 594 affiniPure Donkey Anti-Rat IgG(H + L) Jackson Cat# 712-585-150; RRID: AB_2340688 Alex Fluor 647 affiniPure Donkey Anti-Rat IgG(H + L) Jackson Cat# 712-605-150; RRID: AB_2340693 Anti-Mouse IgG, HRP-linked antibody Cell Signaling Technology Cat# 7076S; RRID: AB_330924 Anti-Rabbit IgG, HRP-linked antibody Cell Signaling Technology Cat# 7074S; RRID: AB_2099233 Anti-Goat IgG, HRP-linked antibody Santa Cruz Cat# sc-2354; RRID: AB_628490 Anti-Mouse IgG for IP, HRP-linked antibody Abcam Cat# ab131368; RRID: AB_2895114 Anti-Goat IgG for IP, HRP-linked antibody Abcam Cat# ab157532; RRID: AB_3676695 Bacterial and virus strains AAV-hLCN2 GeneChem N/A AAV-Gfap-Cre GeneChem N/A AAV-Lcn2 GeneChem N/A Lrp2-shRNA GeneChem N/A Perk-shRNA GeneChem N/A AAV-Lcn2(Δ2-20) GeneChem N/A LV-Lcn2(Δ2-20) GeneChem N/A LV-Lcn2 GeneChem N/A Chemicals, peptides, and recombinant proteins TRIzol Sigma Cat# T9424 DMEM/F12 Gibco Cat# 2749772 Poly-D-Lysine sigma Cat# P6407 L-glutamine Gibco Cat# 35050061 Penicillin-streptomycin Gibco Cat# 15140122 Fetal Bovine Serum Gibco Cat# A5669701 LPS Sigma Cat# L4391-1MG ITSS Roche Cat# 11074547001 PDGF-BB Biolegend Cat# 558804 b-FGF Biolegend Cat# 579604 BSA Sigma Cat# A1193 CNTF PROSPEC Cat# CYT-139 Protease inhibitor cocktail Beyotime Cat# P1006 protein A/G agarose Santa Cruz Cat# sc-2003 CCK-8 kit Beyotime Cat# C0037 LCN2 Elisa kit Boster biological technology Cat# EK0854 (Continued on next page) 20 Cell Reports 44, 115899, July 22, 2025

    Techniques: Knockdown, Expressing, Cell Culture